Journal article
A Mechanism for Sustained Cellulose Synthesis during Salt Stress
A Endler, C Kesten, R Schneider, Y Zhang, A Ivakov, A Froehlich, N Funke, S Persson
Cell | Published : 2015
Abstract
Abiotic stress, such as salinity, drought, and cold, causes detrimental yield losses for all major plant crop species. Understanding mechanisms that improve plants' ability to produce biomass, which largely is constituted by the plant cell wall, is therefore of upmost importance for agricultural activities. Cellulose is a principal component of the cell wall and is synthesized by microtubule-guided cellulose synthase enzymes at the plasma membrane. Here, we identified two components of the cellulose synthase complex, which we call companion of cellulose synthase (CC) proteins. The cytoplasmic tails of these membrane proteins bind to microtubules and promote microtubule dynamics. This activit..
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Awarded by Appalachian Regional Commission
Funding Acknowledgements
D. melanogaster kinesin-1 heavy chain (DmKHC) was kindly provided by Prof. Stefan Diez, B CUBE, Dresden (Germany). Vector pETM11-SUMO3GFP was kindly provided by Dr. Huseyin Besir, European Molecular Biology Laboratory, Heidelberg (Germany). We thank Drs. Clara Sanchez-Rodriguez and Heather McFarlane for useful comments, Dr. Martin Bringmann for help in cloning the truncated CC1 protein, and Drs. Luisa Trindade and Nasim Mansoori for aid with split-ubiquitin assays. Protein preparation was done in cooperation with Martina Leidert and Anne Diehl (FMP Berlin Germany) with vector kindly provided by Patrick Loll (Drexel University Philadelphia, USA). The authors are grateful to the Max-Planck Gesellschaft for funding. C.K. was funded from an IMPRS fellowship via the MPG. Part of the research was funded through the DFG grant PE1642/6-1 and the ARC grant DP150103495.